(Bloy cytometry, fcm) bu onye nyocha sel na-eme nke fluorocence nke akara akwara cell. Ọ bụ teknụzụ ọgbara dị elu mepụtara dabere na nyocha na idozi mkpụrụ ndụ. O nwere ike iji ngwa ngwa tụọ ya ma na-emepụta ogo, nke ime ụlọ, DNA, protein, proteins, ndị na - amu amu na mkpụrụ ndụ ma ọ bụ kemịkalụ ndị ọzọ na kemịkalụ ndị ọzọ, ma nwee ike ịdabere na nchịkọta ndị a.
Fytometers na-eto eto karịa na akụkụ ise ndị a:
1 Flambber na usoro flaịnụ
2 Laser Light Isi mmalite
3 Sistem
4 Eletrọnics, Nchekwa, Ngosipụta na Usoro nyocha
Sistemụ cellt cell
N'etiti ha, laser na-atọ ụtọ na Liter Light Isi mmalite na Sty na-akpụ akpụ bụ ihe dị mkpa maka ngụkọta ngwọta na-asọ asọ. Ike nke ìhè na-atọ ụtọ na oge ikpughe metụtara ike nke radionence. Laser bụ isi iyi ọkụ nke nwere ike inye ọkụ na-agbanye, dị elu, na steepụ Ìhè. Ọ bụ isi iyi egwu kachasị mkpa iji mezuo ihe ndị a.
E nwere oghere cylindrical abụọ n'etiti ebe a na-agagharị na-aga n'ihu. Anya mmiri ndị a na-elekwasị anya na ngalaba okirikiri na-etinye site na ebe a na-eme ihe na-acha odo odo na-enwe ọgbụgbọ ellipical na ngalaba pere mpe (22 μm × 66 × 66 × 66 × 66 × 66 × 66 × 66 × 66 × 66 × 66 × 66 × 66 A na-ekesa ike laser a dị ka ekesara ya dị ka nkesa dị iche iche si kwuo, na-agbanwe ìhè na-agbanwe agbanwe na mkpụrụ ndụ na-agafe laser. N'aka nke ọzọ, usoro anya mejupụtara ọtụtụ oghere, pinholes, na nzacha, nke nwere ike kewara abụọ: elu na ndagide na-agbadata nke ụlọ.
Usoro ngwa anya n'ihu ụlọ nke na-asọpụta nwere oghere na pinhole. Ọrụ bụ isi nke oghere na PINHOLE (na-abụkarị oghere abụọ na PINHOLE) bụ iji lekọta okporo osisi laser site na mpaghara Laser na-eweta nke ọma na ngalaba dị obere. Nke a na-ekesara ikike laser dị ka nkesa ọfụma si dị, na-agba mbọ gbanwee ike na-agbanwe agbanwe maka mkpụrụ osisi nchọpụta laser ma belata nnyonye anya site na ìhè si na-eme mkpọtụ.
E nwere ụdị nzacha atọ dị mkpa:
1: Ogologo Ngalaba Seard (LPF) - Na-enye ohere ọkụ na ebili mmiri dị elu karịa uru a kapịrị ọnụ gabiga.
2: Nchọpụta dị mkpụmkpụ (SPF) - naanị na-enye ohere ọkụ na ebili mmiri n'okpuru otu uru a kapịrị ọnụ gabiga.
3: Ntụgharị Bank (BPF) - Nanị na-enye ohere ọkụ n'akụkụ otu ụdị ụgbọ mmiri gafere.
Nchikota di iche iche nke nzacha nwere ike isi na-egosiputa ihe nlere anya na uzo di iche iche na photomulliles (PMT). Dịka ọmụmaatụ, ihe nzacha maka ịchọpụta fluorecence akwụkwọ ndụ akwụkwọ ndụ (Facc) n'ihu PMT bụ LPF550 na BPF525. Ihe nzacha a na-achọpụta na-acha uhie uhie-acha ọbara ọbara (pe) n'ihu PMT bụ LPF600 na BPF575. Ihe nzacha ahụ maka ịchọpụta fluorescence (Cy5) n'ihu PMT bụ LPF650 na BPF675.
A na-ejikarị cytometry na-asọ asọ. Site n'ọganihu nke teknụzụ kọmputa, mmepe nke immunology teknụzụ monoconal, ngwa ya na bayoloji, ọgwụ na mpaghara ndị ọzọ na-aghọwanye mgbasa. Ngwa ndị a gụnyere nyocha sel, selpoptosis, mkpụrụ ndụ na-egosipụta, nyocha nke ọgwụ na nyocha ọgwụ, wdg.
Oge Post: Sepp-21-2023
