(Flow cytometry, FCM) bụ ihe nyocha mkpụrụ ndụ nke na-atụle ike ọkụ nke ihe nrịbama cell nwere ntụpọ. Ọ bụ teknụzụ ọgbara ọhụrụ mepụtara dabere na nyocha na nhazi nke otu mkpụrụ ndụ. Ọ nwere ike tụọ ngwa ngwa ma kewaa nha, nhazi ime, DNA, RNA, proteins, antigens na ihe ndị ọzọ na-ahụ maka anụ ahụ ma ọ bụ kemịkalụ nke mkpụrụ ndụ, ma nwee ike ịdabere na nchịkọta nke nhazi ndị a.
Cytometer na-efe efe na-enwekarị akụkụ ise ndị a:
1 Flow chamber and fluidics system
2 Isi iyi ọkụ Laser na usoro nhazi osisi
3 Sistemụ ngwa anya
4 Electronics, nchekwa, ngosi na usoro nyocha
5 Sistemụ nhazi mkpụrụ ndụ
N'ime ha, mkpali laser na isi iyi ọkụ laser na sistem na-akpụ ọkụ bụ isi nha nke akara fluorescence na cytometry eruba. Ike nke ọkụ na-akpali akpali na oge ikpughe na-ejikọta ike nke mgbaàmà fluorescence. Laser bụ isi iyi ọkụ na-ejikọta ọnụ nke nwere ike inye ìhè otu ogologo ogologo, ike dị elu na nke dị elu. Ọ bụ isi iyi ọkụ na-akpali akpali iji mezuo ihe ndị a chọrọ.
Enwere lenses cylindrical abụọ n'etiti isi iyi laser na ọnụ ụlọ eruba. Igwe anya m ndị a na-elekwasị anya n'osisi laser nwere akụkụ okirikiri nke na-esi na isi iyi laser banye n'ime oghere elliptical nwere obere akụkụ obe (22 μm × 66 μm). A na-ekesa ike laser n'ime oghere elliptical a dị ka nkesa nkịtị si dị, na-eme ka ọkụ ọkụ na-agbanwe agbanwe maka mkpụrụ ndụ na-agafe na mpaghara nchọpụta laser. N'aka nke ọzọ, usoro ngwa anya nwere ọtụtụ oghere nke lenses, pinholes, na nzacha, nke nwere ike kewaa ya n'ime otu abụọ: elu na mgbada nke ọnụ ụlọ.
Usoro ngwa anya n'ihu ụlọ na-asọpụta nwere oghere na pinhole. Isi ọrụ nke oghere na pinhole (na-abụkarị oghere abụọ na pinhole) bụ ilekwasị anya n'ọkụ laser nwere akụkụ okirikiri okirikiri nke laser na-ewepụta n'ime oghere elliptical nwere obere akụkụ obe. Nke a na-ekesa ike laser dịka nkesa nkịtị si dị, na-eme ka ọkụ ọkụ na-agbanwe agbanwe maka sel n'ofe mpaghara nchọpụta laser na ibelata nnyonye anya site na ìhè kpafuo.
Enwere ụdị nzacha atọ bụ isi:
1: Long pass filter (LPF) - naanị na-enye ohere ka ìhè nwere ogologo ebili mmiri dị elu karịa otu uru ọ ga-agafe.
2: Short-pass filter (SPF) - na-enye ohere ka ìhè nwere ogologo ogologo n'okpuru otu uru gafere.
3: Bandpass filter (BPF) - naanị na-enye ohere ka ìhè dị na ogologo ogologo ogologo gafere.
Ngwakọta nzacha dị iche iche nwere ike iduzi mgbama fluorescence n'ogologo dị iche iche gaa na tubes fotomultiplier (PMTs). Dịka ọmụmaatụ, ihe nzacha maka ịchọpụta fluorescence green (FITC) n'ihu PMT bụ LPF550 na BPF525. Ihe nzacha eji achọpụta fluorescence na-acha ọbara ọbara (PE) n'ihu PMT bụ LPF600 na BPF575. Ihe nzacha maka ịchọpụta fluorescence uhie (CY5) n'ihu PMT bụ LPF650 na BPF675.
A na-ejikarị cytometry na-asọ asọ maka nhazi cell. Site na ọganihu nke teknụzụ kọmputa, mmepe nke immunology na mmepụta nke teknụzụ antibody monoclonal, ngwa ya na bayoloji, ọgwụ, ụlọ ahịa ọgwụ na mpaghara ndị ọzọ na-aghọwanye ebe niile. Ngwa ndị a gụnyere nyocha mgbanwe mkpụrụ ndụ, apoptosis cell, mpịnye cell, nchọpụta etuto, nyocha nrụpụta ọgwụ, wdg.
Oge nzipu: Sep-21-2023
